This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (70) Citing Articles via Google Scholar Google Scholar Articles by Owicki, J. C. Search for Related Content PubMed PubMed Citation Articles by Owicki, J. C. Social Bookmarking                   What's this?

Fluorescence Polarization and Anisotropy in High Throughput Screening: Perspectives and Primer

Palo Alto, CA

Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to construct homogeneous assays that are fast, sensitive, and resistant to some significant interferences. Moreover, the assays are relatively inexpensive. This review surveys the peer-reviewed literature on the subject and explores some of the fundamental issues that bear on assay performance.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J.-P. Luzy, Huixiong Chen, B. Gril, W.-Q. Liu, M. Vidal, D. Perdereau, A.-F. Burnol, and C. Garbay Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization J Biomol Screen, February 1, 2008; 13(2): 112 - 119. [Abstract] [PDF]

				K. L. Huss, P. E. Blonigen, and R. M. Campbell Development of a TranscreenerTM Kinase Assay for Protein Kinase A and Demonstration of Concordance of Data with a Filter-Binding Assay Format J Biomol Screen, June 1, 2007; 12(4): 578 - 584. [Abstract] [PDF]

				O. Nosjean, S. Souchaud, C. Deniau, O. Geneste, N. Cauquil, and J. A. Boutin A Simple Theoretical Model for Fluorescence Polarization Binding Assay Development J Biomol Screen, December 1, 2006; 11(8): 949 - 958. [Abstract] [PDF]

				E. M. August, L. Patnaude, J. Hopkins, J. Studts, E. Gautschi, A. Shrutkowski, A. Kronkaitis, M. Brown, A. Kabcenell, and D. Rajotte Development of a High-Throughput Assay to Measure Histidine Decarboxylase Activity J Biomol Screen, October 1, 2006; 11(7): 816 - 821. [Abstract] [PDF]

				Y. Du, S. C. Masters, F. R. Khuri, and H. Fu Monitoring 14-3-3 Protein Interactions with a Homogeneous Fluorescence Polarization Assay J Biomol Screen, April 1, 2006; 11(3): 269 - 276. [Abstract] [PDF]

				M. J. Nicholson, B. Moradi, N. P. Seth, X. Xing, G. D. Cuny, R. L. Stein, and K. W. Wucherpfennig Small Molecules That Enhance the Catalytic Efficiency of HLA-DM J. Immunol., April 1, 2006; 176(7): 4208 - 4220. [Abstract] [Full Text] [PDF]

				B. K. Hoefelschweiger, L. Pfeifer, and O. S. Wolfbeis Screening Scheme Based on Measurement of Fluorescence Lifetime in the Nanosecond Domain J Biomol Screen, October 1, 2005; 10(7): 687 - 694. [Abstract] [PDF]

				S. M. Miick, S. Jalali, B. P. Dwyer, J. Havens, D. Thomas, M. A. Jimenez, M. T. Simpson, B. Zile, K. L. Huss, and R. M. Campbell Development of a Microplate-Based, Electrophoretic Fluorescent Protein Kinase A Assay: Comparison with Filter-Binding and Fluorescence Polarization Assay Formats J Biomol Screen, June 1, 2005; 10(4): 329 - 338. [Abstract] [PDF]

				M. Newman and S. Josiah Utilization of Fluorescence Polarization and Time Resolved Fluorescence Resonance Energy Transfer Assay Formats for SAR Studies: Src Kinase as a Model System J Biomol Screen, September 1, 2004; 9(6): 525 - 532. [Abstract] [PDF]

				B. Ason and W. S. Reznikoff A high-throughput assay for Tn5 Tnp-induced DNA cleavage Nucleic Acids Res., June 16, 2004; 32(10): e83 - e83. [Abstract] [Full Text] [PDF]

				E. A. Gaudet, K.-S. Huang, Y. Zhang, W. Huang, D. Mark, and J. R. Sportsman A Homogeneous Fluorescence Polarization Assay Adaptable for a Range of Protein Serine/Threonine and Tyrosine Kinases J Biomol Screen, April 1, 2003; 8(2): 164 - 175. [Abstract] [PDF]

				T. C. Turek-Etienne, E. C. Small, S. C. Soh, T. A. Xin, P. V. Gaitonde, E. B. Barrabee, R. F. Hart, and R. W. Bryant Evaluation of Fluorescent Compound Interference in 4 Fluorescence Polarization Assays: 2 Kinases, 1 Protease, and 1 Phosphatase J Biomol Screen, April 1, 2003; 8(2): 176 - 184. [Abstract] [PDF]

				S. Bollini, J. J. Herbst, G. T. Gaughan, T. A. Verdoorn, J. Ditta, G. M. Dubowchik, and A. Vinitsky High-Throughput Fluorescence Polarization Method for Identification of FKBP12 Ligands J Biomol Screen, December 1, 2002; 7(6): 526 - 530. [Abstract] [PDF]

				P. A. Wright, H. F. Boyd, R. C. Bethell, M. Busch, P. Gribbon, J. Kraemer, E. Lopez-Calle, T. H. Mander, D. Winkler, and N. Benson Development of a 1-{micro}l Scale Assay for Mitogen-Activated Kinase Kinase 7 Using 2-D Fluorescence Intensity Distribution Analysis Anisotropy J Biomol Screen, October 1, 2002; 7(5): 419 - 428. [Abstract] [PDF]

				H. Flotow, C.-Y. Leong, and A. D. Buss Development of a Plasmepsin II Fluorescence Polarization Assay Suitable for High Throughput Antimalarial Drug Discovery J Biomol Screen, August 1, 2002; 7(4): 367 - 371. [Abstract] [PDF]

				M. A. Sills, D. Weiss, Q. Pham, R. Schweitzer, X. Wu, and J. J. Wu Comparison of Assay Technologies for a Tyrosine Kinase Assay Generates Different Results in High Throughput Screening J Biomol Screen, June 1, 2002; 7(3): 191 - 214. [Abstract] [PDF]

				W. Huang, Y. Zhang, and J. R. Sportsman A Fluorescence Polarization Assay for Cyclic Nucleotide Phosphodiesterases J Biomol Screen, June 1, 2002; 7(3): 215 - 222. [Abstract] [PDF]

				P. Banks and M. Harvey Considerations for Using Fluorescence Polarization in the Screening of G Protein-Coupled Receptors J Biomol Screen, April 1, 2002; 7(2): 111 - 117. [Abstract] [PDF]

				M. Allen, D. Hall, B. Collins, and K. Moore A Homogeneous High Throughput Nonradioactive Method for Measurement of Functional Activity of Gs-Coupled Receptors in Membranes J Biomol Screen, February 1, 2002; 7(1): 35 - 44. [Abstract] [PDF]

				S. Turconi, K. Shea, S. Ashman, K. Fantom, D. L. Earnshaw, R. P. Bingham, U. M. Haupts, M. J.B. Brown, and A. J. Pope Real Experiences of uHTS: A Prototypic 1536-Well Fluorescence Anisotropy-Based uHTS Screen and Application of Well-Level Quality Control Procedures J Biomol Screen, October 1, 2001; 6(5): 275 - 290. [Abstract] [PDF]

				L. Prystay, M. Gosselin, and P. Banks Determination of Equilibrium Dissociation Constants in Fluorescence Polarization J Biomol Screen, June 1, 2001; 6(3): 141 - 150. [Abstract] [PDF]