This Article Full Text (OnlineFirst PDF) All Versions of this Article: 1087057108330111v1 14/3/219    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Baugh, C. Articles by Thompson, P. A. Search for Related Content PubMed PubMed Citation Articles by Baugh, C. Articles by Thompson, P. A. Social Bookmarking                         What's this?

SCAN^TM—A High-Throughput Assay for Detecting Small Molecule Binding to RNA Targets

^* To whom correspondence should be addressed. E-mail: pthompson{at}anadyspharma.com.

	Abstract

A novel optical-based high-throughput screening technology has been developed for increasing the rate of discovering chemical leads against RNA targets. SCAN^TM (Screen for Compounds with Affinity for Nucleic acids) is an affinity-based assay that identifies small molecules that bind and recognize structured RNA elements. This technology provides the opportunity to conduct high-throughput screening of a new class of targets—RNA. SCAN^TM offers many attractive features including a simple homogeneous format, low screening costs, and the ability to use common laboratory equipment. A SCAN^TM assay was developed for the HCV IRES Loop IIId RNA domain. A high-throughput screen of our entire compound library resulted in the identification of small molecule ligands that bind to Loop IIId. The Z' values were greater than 0.8, showing this to be a robust high-throughput screening assay. A correlation between SCAN^TM EC₅₀ and K_D values is reported suggesting the ability to use the assay for compound optimization. (Journal of Biomolecular Screening XXXX:xx-xx)

First published on February 11, 2009, doi:10.1177/1087057108330111

Journal of Biomolecular Screening 2009;14:219.

A more recent version of this article appeared on March 1, 2009


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?