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Journal of Biomolecular Screening
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Article

High-Throughput Analysis of HGF-Stimulated Cell Scattering

Grace K. Y. Chan, Bart A. Lutterbach, Bo-Sheng Pan, Ilona Kariv, and Alexander A. Szewczak*

* To whom correspondence should be addressed. E-mail: alexander_szewczak{at}merck.com.


  Abstract
Historically, only relatively low-throughput or expensive methods have been available to measure cell migration. Hepatocyte growth factor (HGF) is a ligand for the tyrosine kinase receptor Met that, in addition to mediating proliferation and survival, increases cell motility and metastasis. The authors have developed a high-throughput imaging assay for measuring inhibition of HGF-induced scattering in human HPAF-II pancreatic adenocarcinoma cells. Following treatment with test compounds and HGF for 24 h, cells are labeled with a nuclear stain and imaged at 10x magnification. The proximity of neighboring nuclei is measured, and the distribution of internuclear distances across each field of view is used to calculate the fraction of scattered cells. This method of analysis can be extended to other cell types and signaling pathways and, compared with other membrane-based migration assays currently available, the assay is significantly lower in cost, is less labor intensive, and provides higher throughput. (Journal of Biomolecular Screening XXXX:xx-xx)

First published on September 23, 2008, doi:10.1177/1087057108324497

Journal of Biomolecular Screening 2008;13:847.

A more recent version of this article appeared on October 1, 2008

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