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Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe

Guangxi Normal University

^* To whom correspondence should be addressed. E-mail: zljiang{at}mailbox.gxnu.edu.cn.

	Abstract

Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH₂PO₄Na₂HPO₄ buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm (I_{580 nm}) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity. (Journal of Biomolecular Screening XXXX:xx-xx)

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