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Journal of Biomolecular Screening
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Article

Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe

Zhiliang Jiang*, Lili Wei, Mingjing Zou, Aihui Liang, and Mianwu Meng

Guangxi Normal University

* To whom correspondence should be addressed. E-mail: zljiang{at}mailbox.gxnu.edu.cn.


  Abstract
Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH2PO4Na2HPO4 buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm ({Delta}I580 nm) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity. (Journal of Biomolecular Screening XXXX:xx-xx)

First published on March 18, 2008, doi:10.1177/1087057108316737

Journal of Biomolecular Screening 2008;13:302.

A more recent version of this article appeared on April 1, 2008

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