This Article Full Text (OnlineFirst PDF) All Versions of this Article: 1087057108315513v1 13/3/194    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ning, B. Articles by Guo, L. Search for Related Content PubMed PubMed Citation Articles by Ning, B. Articles by Guo, L. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB 3-METHYLCHOLANTHRENE OMEPRAZOLE RIFAMPIN Social Bookmarking                         What's this?

Systematic and Simultaneous Gene Profiling of 84 Drug-Metabolizing Genes in Primary Human Hepatocytes

NCTR

^* To whom correspondence should be addressed. E-mail: lei.guo{at}fda.hhs.gov.

	Abstract

Drug-metabolizing enzymes are an important battery of proteins that are involved in drug metabolism, xenobiotic detoxification, and drug-induced toxicity. Systematic, efficient, and simultaneous evaluation of drug-metabolizing gene expression in response to chemicals has a wide variety of implications in drug development, disease prevention, and personalized medicine and nutrition. In the current study, the authors have systematically and simultaneously evaluated the hepatic expression profile of drug-metabolizing enzymes in cultured human hepatocytes exposed to the xenobiotics rifampicin, omeprazole, and 3-methylcholanthrene (3-MC) using the Drug Metabolism RT²Profiler^TM PCR Arrays. This new high-throughput tool allowed the authors to evaluate the expression of genes coding for 84 drug-metabolizing enzymes (including phase 1 and phase 2 drug-metabolizing enzymes and transporters) simultaneously, in a 96-well format using a small amount of experimental materials. To validate the quality of the Drug Metabolism RT²Profiler^TM PCR Arrays, the PCR Array was compared with the well-documented platform TaqMan assay, and a high concordance was shown between these 2 methods, indicating the high reliability of the Drug Metabolism RT²Profiler^TM PCR Arrays. In addition, increasing or decreasing the expression of drug-metabolizing enzymes by these 3 compounds was observed, and underlying mechanisms are discussed. (Journal of Biomolecular ScreeningXXXX;xx-xx)

First published on February 12, 2008, doi:10.1177/1087057108315513

Journal of Biomolecular Screening 2008;13:194.

A more recent version of this article appeared on March 1, 2008


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				R. M. Sawaya and D. S. Riddick Cytochrome P450 2C11 5'-Flanking Region and Promoter Mediate in Vivo Suppression by 3-Methylcholanthrene Drug Metab. Dispos., September 1, 2008; 36(9): 1803 - 1811. [Abstract] [Full Text] [PDF]