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Journal of Biomolecular Screening
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Article

Rapid Screen of Human Genes for Relevance to Cancer Using Fission Yeast

Kyung-Sook Chung, Young-Joo Jang, Nam-Soon Kim, Sun-Yong Park, Shin-Jung Choi, Ji-Youn Kim, Ji-Hee Ahn, Hyun-Ji Lee, Ji-Hyun Lim, Ju-Hyun Song, Jae-Hoon Ji, Jung-Hwa Oh, Kyung-Bin Song, Hyang-Sook Yoo, Misun Won*

* To whom correspondence should be addressed. E-mail: misun{at}kribb.re.kr.


  Abstract
A total of 437 human full-length cDNAs isolated by microarray analysis of liver and/or gastric cancer tissues were evaluated for their relevance to cancer using the fission yeast Schizosaccharomyces pombe. Overexpression of 161 human cDNAs in S. pombe caused growth inhibition and/or morphological changes, which can be considered as cancer-related phenotypes of S. pombe. Sixteen genes causing growth defects and morphological changes at the same time were chosen to validate their ostensible oncogenic properties. They were highly expressed in liver and/or gastric cancer cell lines. Also, when the mouse embryonic fibroblast cell type NIH3T3 was transfected with these genes, the proliferation rates of cells were increased by 32% to 120%. This study demonstrates that fission yeast can be used as an advantageous and powerful tool for the rapid screening of human genes relevant to cancer. Furthermore, the human genes screened can be tested further as diagnostic markers and potential therapeutic targets for liver and stomach cancers. They also can be studied further for the elucidation of mechanisms involved in carcinogenesis. (Journal of Biomolecular Screening XXXX:xx-xx)

First published on May 3, 2007, doi:10.1177/1087057107301007

Journal of Biomolecular Screening 2007;12:568.

A more recent version of this article appeared on June 1, 2007

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