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Journal of Biomolecular Screening
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1087057107300221v1
12/4/578    most recent
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Article

Development of a TranscreenerTM Kinase Assay for Protein Kinase A and Demonstration of Concordance of Data with a Filter-Binding Assay Format

Karen L. Huss*, Pauline E. Blonigen, Robert M. Campbell

* To whom correspondence should be addressed. E-mail: Huss_Karen_L{at}Lilly.com.


  Abstract
A TranscreenerTM kinase fluorescence polarization (FP) assay has been developed for the serine/threonine kinase protein kinase A (PKA). The PKA TranscreenerTM kinase assay is an homogenous, competitive antibody-based FP assay that uses Far Red Alexa Fluor 633-labeled adenosine 5' disphosphate (ADP) tracer and mouse monoclonal anti-ADP antibody. The TranscreenerTM PKA assay was validated with both known PKA inhibitors and library compounds. The TranscreenerTM PKA assay is resistant to low-wavelength (or common) fluorescent interference from small-molecule library compounds and generates IC50 results comparable with current radioactive filter-binding assay. (Journal of Biomolecular Screening XXXX:xx-xx)

First published on April 4, 2007, doi:10.1177/1087057107300221

Journal of Biomolecular Screening 2007;12:578.

A more recent version of this article appeared on June 1, 2007

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