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Journal of Biomolecular Screening
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Article

A Comparison of Assay Performance Measures in Screening Assays: Signal Window, Z' Factor, and Assay Variability Ratio

Philip W. Iversen*, Brian J. Eastwood, G. Sitta Sittampalam, Karen L. Cox

Lilly Research Laboratories, Eli Lilly & Company, Indianapolis, Indiana.

* To whom correspondence should be addressed. E-mail: piversen{at}lilly.com.


  Abstract

In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They examine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a computer simulation, and illustrate their use with example data. Based on these results, the authors recommend the Z' factor as a preferred measure of assay performance for screening assays and point out that none of these measures are adequate for characterizing concentration-response assays.

Key Words: signal window, Z' factor, assay variability ratio, assay validation

First published on February 20, 2006, doi:10.1177/1087057105285610

Journal of Biomolecular Screening 2006;11:247.

A more recent version of this article appeared on April 1, 2006

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