| Sign In to gain access to subscriptions and/or personal tools. |
NanoStore: A Concept for Logistical Improvements of Compound Handling in High-Throughput Screening
1 Discovery Biology, Pfizer Global Research and Development, Ramsgate Road, Sandwich, CT13 9NJ, United Kingdom.
* To whom correspondence should be addressed. E-mail: r_eryl_sharp{at}groton.pfizer.com.
Small molecule screening, the systematic encounter of biology space with chemical space, has provoked the emergence of a whole industry that recreates itself by constant iterative improvements to this process. The authors describe an approach to tackle the problem for one of the most time-consuming steps in the execution of a screening campaign, namely, the reformatting of high-throughput screening test compounds from master plates to daughter assay plates used in the execution of the screen. Through an engineered storage procedure, they prepare plates ahead of the screening process with the respective compounds in a ready-to-use format. They show the biological inertness of the method and how it facilitates efficient recovery of compound activity. This uncoupling of normally interconnected processes provides time and compound savings, avoids repeated freeze-thaw cycles of compound solutions, and removes the problems associated with the DMSO sensitivity of certain assays types. Key Words: assay miniaturization, ultra-high-throughput screening, compound storage and recovery, 1536-well and 2080-well NanoCarriersTM
First published on June 24, 2005, doi:10.1177/1087057105277234 This article has been cited by other articles:
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 |
|
|
 |
|
Sign In to gain access to subscriptions and/or personal tools.
