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High-Throughput Screening for Antimicrobial Compounds Using a 96-Well Format Bacterial Motility Absorbance Assay

Biological Sciences, Western Michigan University, Kalamazoo

Albert A. Barrese

Biological Sciences, Western Michigan University, Kalamazoo

Brian C. Tripp

Biological Sciences, Western Michigan University, Kalamazoo

Brian C. Tripp

Biological Sciences Western Michigan University Mailstop 5410, 1903 West Michigan Avenue Kalamazoo, MI 49008-5410, brian.tripp{at}wmich.edu

There is a pressing need to develop new antimicrobial drugs because of the increasing resistance of pathogenic bacteria to existing antibiotics. The preliminary development and validation of a novel methodology for the high-throughput screening of antimicrobial compounds and inhibitors of bacterial motility is described. This method uses a bacterial motility swarming agar assay, combined with the use of offset inoculation of the wells in a standard, clear, 96-well plate, to enable rapid screening of compounds for potential antibiotic and antimotility properties with a standard absorbance microplate reader. Thus, the methodology should be compatible with 96-well laboratory automation technology used in drug discovery and chemical biology studies. To validate the screening method, the Genesis Plus structurally diverse library of 960 biologically active compounds was screened against a motile strain of the gram-negative bacterial pathogen Salmonella typhimurium. The average Z' value for the positive and negative motility controls on all 12 compound plates was 0.67 ± 0.14, and the signal-to-baseline ratio calculated from the positive and negative controls was 5.9 ± 1.1. A collection of 70 compounds with well-known antimicrobial properties was successfully identified using this assay. (Journal of Biomolecular Screening 2007:849-854)

Key Words: Salmonella • motility assay • high-throughput screening • antimicrobial • 96-well plate

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This version was published on September 1, 2007

Journal of Biomolecular Screening, Vol. 12, No. 6, 849-854 (2007)
DOI: 10.1177/1087057107304478


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Malapaka, V. R.R. Articles by Tripp, B. C. Search for Related Content PubMed PubMed Citation Articles by Malapaka, V. R.R. Articles by Tripp, B. C. Pubmed/NCBI databases Medline Plus Health Information Antibiotics Social Bookmarking                         What's this?