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This version was published on September 1, 2006
Journal of Biomolecular Screening, Vol. 11, No. 6, 704-711 (2006)
DOI: 10.1177/1087057106289288

Automated Agar Plate Streaker: A Linear Plater on Society for Biomolecular Sciences Standard Plates

Gregory W. King

Department of Research Operations, Merck Research Laboratories, Rahway, New Jersey

Gary S. Kath

Department of Research Operations, Merck Research Laboratories, Rahway, New Jersey

Sal Siciliano

Department of Research Operations, Merck Research Laboratories, Rahway, New Jersey

Neal Simpson

Department of Research Operations, Merck Research Laboratories, Rahway, New Jersey

Prakash Masurekar

Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Rahway, New Jersey

Jan Sigmund

Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Rahway, New Jersey

Jon Polishook

Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Rahway, New Jersey

Stephen Skwish

Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Rahway, New Jersey

Gerald Bills

Centro de Investigación Basica, Merck Sharp & Dohme de España, Madrid, Spain

Olga Genilloud

Centro de Investigación Basica, Merck Sharp & Dohme de España, Madrid, Spain

Fernando Peláez

Centro de Investigación Basica, Merck Sharp & Dohme de España, Madrid, Spain

Jesus Martín

Department of Research Operations, Merck Research Laboratories, Rahway, New Jersey

Claude Dufresne

Department of Research Operations, Merck Research Laboratories, Rahway, New Jersey

Several protocols for bacterial isolation and techniques for aerobic plate counting rely on the use of a spiral plater to deposit concentration gradients of microbial suspensions onto a circular agar plate to isolate colony growth. The advantage of applying a gradient of concentrations across the agar surface is that the original microbiological sample can be applied at a single concentration rather than as multiple serial dilutions. The spiral plater gradually dilutes the sample across a compact area and therefore saves time preparing dilutions and multiple agar plates. Commercial spiral platers are not automated and require manual sample loading. Dispensing of the sample volume and rate of gradients are often very limited in range. Furthermore, the spiral sample application cannot be used with rectangular microplates. Another limitation of commercial spiral platers is that they are useful only for dilute, filtered suspensions and cannot plate suspensions of coarse organic particles therefore precluding the use of many kinds of microorganism-containing substrata. An automated agar plate spreader capable of processing 99 rectangular microplates in unattended mode is described. This novel instrument is capable of dispensing discrete volumes of sample in a linear pattern. It can be programmed to dispense a sample suspense at a uniform application rate or across a decreasing concentration gradient.

Key Words: agar • microbial • linear • plater

References


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This Article
Right arrow Abstract Freely available
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Right arrow Citing Articles via ISI Web of Science (1)
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Right arrow Articles by King, G. W.
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PubMed
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Right arrow Articles by King, G. W.
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