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An Improved Method for Checking HTS/uHTS Liquid-Handling Systems
Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, Jena, Germany.
CyBio AG, Jena, Germany
Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, Jena, Germany. An efficient method is presented to determine precision and accuracy of multichannel liquid-handling systems under conditions near to application. Themethod consists of gravimetrical determination of accuracy and optical determination of precision based on the dilution of absorbing and fluorescent dye solutions in microplates. Mean delivery volume per well can be determined with precision better than a 0.04% coefficient of variation (CV). Optical signal precision, CV(S), is improved by multiwavelength measurements. Precision of absorbance measurement yields a better resolution than precision of fluorescence measurement (0.3% and 1.5%, respectively), indicating that absorbance measurements should be preferred. From CV(S), an upper bound of the precision of the volumes delivered is derived. Method performance is demonstrated with the dispenser CyBiTM-Drop and the pipettor CyBiTM-Well using different ejection principles; with commonly used fluids; with 96-, 384-, and 1536-well microplates; and with photometric and fluorometric indicators. Precision of the volumes delivered, as obtained with optimized methods, all plate formats, and both devices, is better than 2% CV with 2 µ L set volume and about 1% CV with higher set volumes.
Key Words: liquid-handling evaluation • microplates • HTS devices • multiwavelength
Journal of Biomolecular Screening, Vol. 9, No. 8,
726-733 (2004) This article has been cited by other articles:
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