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Journal of Biomolecular Screening, Vol. 9, No. 8, 712-718 (2004)
DOI: 10.1177/1087057104269495

A Quantitative High-Throughput Endothelial Cell Migration Assay

Vladimir Mastyugin

Elizabeth McWhinnie

Mark Labow

Novartis Institutes for Biomedical Research, Cambridge, MA.

Frank Buxton

Novartis Institutes for Biomedical Research, 250 Massachusetts Avenue, Cambridge, MAfrank.buxton{at}pharma.novartis.com

By combining the use of BD Biosciences Fluoro Blok TM membrane-based Boyden chambers with the Cellomics HCS Array Scan, a more sensitive method formeasuring cellmigration has been developed. This assay is based on counting nuclei ofmigrated cells on the bottomof the filter rather than conventional approaches, which usemeasurement of totalwell fluorescence. This cellmigration assay provides approximately 10-fold increased signal/background compared to conventional approaches and can be used to assess the effects of growth factors on endothelial cell migration and to screen chemical compounds for inhibitory effects on growth factor–mediated endothelial cell migration.

Key Words: human umbilical vein endothelial cells (HUVEC) • vascular endothelial growth factor (VEGF) • automated cell • migration (ACM)


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