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Journal of Biomolecular Screening
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A High-Throughput Turbidometric Assay for Screening Inhibitors of Protein Disulfide Isomerase Activity

Anthony M. Smith

John Chan

Donna Oksenberg

Roman Urfer

David S. Wexler

Arnie Ow

Liping Gao

Alanna McAlorum

Shu-Gui Huang

Lead Discovery Department, AGY Therapeutics Inc, 270 E. Grand Avenue, South San Francisco, CA 94080, USAfhuang{at}agyinc.com

Protein disulfide isomerase (PDI) plays a key role in protein folding by catalyzing rearrangements of disulfide bonds in substrate proteins following their synthesis in eukaryotic cells. Besides its major role in the processing and maturation of secretory proteins in the endoplasmic reticulum, this enzyme and its homologs have been implicated in multiple important cellular processes; however, they have not served as targets for the development of therapeutic agents. The authors developed a high-throughput screening assay for PDI and its homologous enzymes in 384-well microplates. The method is based on the enzyme-catalyzed reduction of insulin in the presence of dithiothreitol and measures the aggregation of reduced insulin chains at 650 nm. This kinetic assay was converted to an end-point assay by using hydrogen peroxide as a stop reagent. The feasibility of this high-throughput assay for screening chemical libraries was demonstrated in a pilot screen. The authors show that this homogenous turbidometric assay is robust and cost-effective and can be applied to identify PDI inhibitors from chemical libraries, opening this class of enzymes for therapeutic exploration.

Key Words: protein disulfide isomerase • turbidometric assay • reductive cleavage of disulfide bonds • high-throughput screening

Journal of Biomolecular Screening, Vol. 9, No. 7, 614-620 (2004)
DOI: 10.1177/1087057104265292
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