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DOI: 10.1177/1087057104266740 Development of a Colorimetric Method for Functional Chloride Channel Assay1000 Rt 202 South, Pharmaceutical Research and Development, Johnson & Johnson, Raritan, NJ 08869wtang1{at}prdus.jnj.com
Anion channels play significant physiological roles in humans and animals. However, the effort of screening for anion channel modulators was limited by the available assay technologies. This report discusses the development of a cell-based functional chloride channel assay using iodine as the chloride channel functional indicator. Iodine concentrations were measured with modified Sandell-Kolthoff reaction using colorimetric detection. The assay was rapid and quantitative. When WSS-1 cells were activated by
Key Words: chloride channels cell-based assays colorimetric method modified Sandell-Kolthoff reaction functional anion channel assay GABAA receptor CFTR receptor
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