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Journal of Biomolecular Screening
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SpeedScreen: The "Missing Link" between Genomics and Lead Discovery

Hartmut Zehender

Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.208hartmut.zehender{at}pharma.novartis.com

Francois Le Goff

Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland

Natalie Lehmann

Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.209

Ireos Filipuzzi

Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.114

Lorenz M. Mayr

Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.102

SpeedScreen is a novel, label-free, in-solution, affinity-based selection methodology for high-throughput screening (HTS) developed at Novartis Pharma. The SpeedScreen protocol comprises in-solution affinity selection, followed by size exclusion chromatography in combination with microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS). The authors describe the basic concept behind assay development, HTS, and data analysis with the SpeedScreen technology. Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS.

Key Words: affinity selection • mass spectrometry • high-throughput screening • orphan targets • nontractable targets • chemical genomics

Journal of Biomolecular Screening, Vol. 9, No. 6, 498-505 (2004)
DOI: 10.1177/1087057104267605
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