Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to register for free online access

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biomolecular Screening
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Huff, J. L.
Right arrow Articles by Henderson, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huff, J. L.
Right arrow Articles by Henderson, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Label-Free Protein and Pathogen Detection Using the Atomic Force Microscope

Janice L. Huff

BioForce Nanosciences, Inc., Ames, IA; University Space Research Association/Johnson Space Center, Houston, TX

Michael P. Lynch

BioForce Nanosciences, Inc., Ames, IAmlynch{at}bioforcenano.com

Saju Nettikadan

BioForce Nanosciences, Inc., Ames, IA

James C. Johnson

BioForce Nanosciences, Inc., Ames, IA; Des Moines University, Department of Microbiology, Des Moines, IA

Srikanth Vengasandra

BioForce Nanosciences, Inc., Ames, IA

Eric Henderson

BioForce Nanosciences, Inc., Ames, IA; Iowa State University, Department of GDCB, Ames, IA

The atomic force microscope (AFM) uses a sharp micron-scale tip to scan and amplify surface features, providing exceptionally detailed topographical information with magnification on the order of x106. This instrument is used extensively for quality control in the computer and semiconductor industries and is becoming a progressively more important tool in the biological sciences. Advantages of the AFM for biological application include the ability to obtain information in a direct, label-free manner and the ability to image in solution, providing real-time data acquisition under physiologically relevant conditions. A novel application of the AFM currently under development combines its surface profiling capabilities with fixed immuno-capture using antibodies immobilized in a nanoarray format. This provides a distinctive platform for direct, label-free detection and characterization of viral particles and other pathogens.

Key Words: atomic force microscope • nanoarray • virus detection • label-free detection

Journal of Biomolecular Screening, Vol. 9, No. 6, 491-497 (2004)
DOI: 10.1177/1087057104268803
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?