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Journal of Biomolecular Screening
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Label-Free Assays on the BIND System

Brian T. Cunningham

SRU Biosystems, 14A Gill Street, Woburn, Massachusettsbcunningham{at}srubiosystems.com

Peter Li

Stephen Schulz

Bo Lin

Cheryl Baird

John Gerstenmaier

Christine Genick

Frank Wang

Eric Fine

Lance Laing

SRU Biosystems, 14A Gill Street, Woburn, Massachusetts

Screening of biochemical interactions becomes simpler, less expensive, and more accurate when labels, such as fluorescent dyes, radioactive markers, and colorimetric reactions, are not required to quantify detected material. SRU Biosystems has developed a biosensor technology that is manufactured on continuous sheets of plastic film and incorporated into standard microplates and microarray slides to enable label-free assays to be performed with high throughput, high sensitivity, and low cost per assay. The biosensor incorporates a narrow band guided-mode resonance reflectance filter, in which the reflected color is modulated by the attachment/detachment of biochemical material to the surface. The technology offers 4 orders of linear dynamic range and uniformity within a plate, with a coefficient of variation of 2.5%. Using conventional biochemical immobilization surface chemistries, a wide range of assay applications are enabled. Small molecule screening, cell proliferation/cytotoxicity, enzyme activity screening, protein-protein interaction, and cell membrane receptor expression are among the applications demonstrated.

Key Words: Biomolecular Interaction Detection system • BIND • biosensors • pharmaceutical screening • label-free detection

Journal of Biomolecular Screening, Vol. 9, No. 6, 481-490 (2004)
DOI: 10.1177/1087057104267604
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This article has been cited by other articles:


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 J Biomol ScreenHome page
Z. Zhu and J. Cuozzo
Review Article: High-Throughput Affinity-Based Technologies for Small-Molecule Drug Discovery
J Biomol Screen, December 1, 2009; 14(10): 1157 - 1164.
[Abstract] [PDF]