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Journal of Biomolecular Screening
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Oncology Drug Discovery Applications Using the FMATTM 8100 HTS System

Jennifer Y. Lee

Novartis Institutes for Biomedical Research, Cambridge, MA, Presently at Harvard Medical School, Boston

Sheri Miraglia

Applied Biosystems, Foster City, CA

Xiongwei Yan

Applied Biosystems, Foster City, CA

Elana Swartzman

Applied Biosystems, Foster City, CA

Susan Cornell-Kennon

Novartis Institutes for Biomedical Research, Cambridge, MA

Julia Mellentin-Michelotti

Applied Biosystems, Foster City, CA

Charles Bruseo

Novartis Institutes for Biomedical Research, Cambridge, MA, GlaxoSmithKline, Research Triangle Park, NC

Dennis S. France

Novartis Institutes for Biomedical Research, Cambridge, MA, dennis.france{at}pharma.novartis.com

High-throughput screening (HTS) for potential anticancer agents requires a broad portfolio of assay platforms that may include kinase enzyme assays, protein-protein binding assays, and functional cell-based apoptosis assays. The authors have explored the use of fluorometric microvolume assay technology (the FMATTM 8100 HTS System) in three distinct homogeneous HTS assays: (1) a Src tyrosine kinase enzyme assay, (2) a Grb2-SH2 protein-peptide interaction assay, and (3) an annexin V binding apoptosis assay. Data obtained from all three assays suggest that the FMAT system should facilitate the implementation of homogeneous assays for a wide variety of molecular targeted and cell-based screens. (Journal of Biomolecular Screening 2003:81-88)

Key Words: high-throughput screening • oncology • anticancer agents • assay platforms

Journal of Biomolecular Screening, Vol. 8, No. 1, 81-88 (2003)
DOI: 10.1177/1087057102239668
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