This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Taylor, P. B. Articles by Macarron, R. Search for Related Content PubMed PubMed Citation Articles by Taylor, P. B. Articles by Macarron, R. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?

A Standard Operating Procedure for Assessing Liquid Handler Performance in High-Throughput Screening

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Stephen Ashman

Department of Assay Methodology Development, New Frontiers Science Park, Harlow, United Kingdom

Stuart M. Baddeley

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Stacy L. Bartram

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Clive D. Battle

Cheminformatics, King of Prussia, PA and Stevenage, United Kingdom

Brian C. Bond

Department of Statistical Sciences, New Frontiers Science Park, Harlow, United Kingdom

Yvonne M. Clements

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Nathan J. Gaul

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

W. Elliot McAllister

Cheminformatics, King of Prussia, PA and Stevenage, United Kingdom

Juan A. Mostacero

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Fernando Ramon

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Jamie M. Wilson

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Robert P. Hertzberg

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Andrew J. Pope

Systems Research, King of Prussia, PA.

Ricardo Macarron

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

The thrust of early drug discovery in recent years has been toward the configuration of homogeneous miniaturized assays. This has allowed organizations to contain costs in the face of exponential increases in the number of screening assays that need to be run to remain competitive. Miniaturization brings with it an increasing dependence on instrumentation, which over the past several years has seen the development of nanodispensing capability and sophisticated detection strategies. To maintain confidence in the data generated from miniaturized assays, it is critical to ensure that both compounds and reagents have been delivered as expected to the target wells. The authors have developed a standard operating procedure for liquid-handling quality control that has enabled them to evaluate performance on 2 levels. The first level provides for routine daily testing on existing instrumentation, and the second allows for more rigorous testing of new dispensing technologies. The procedure has shown itself to be useful in identifying both method programming and instrumentation performance shortcomings and has provided a means to harmonizing instrumentation usage by assay development and screening groups. The goal is that this type of procedure be used for facilitating the exchange of liquid handler performance data across the industry.

Journal of Biomolecular Screening, Vol. 7, No. 6, 554-569 (2002)
DOI: 10.1177/1087057102238630


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				I. Popa-Burke, B. Lupotsky, J. Boyer, W. Gannon, R. Hughes, P. Kadwill, D. Lyerly, J. Nichols, E. Nixon, D. Rimmer, et al. Establishing Quality Assurance Criteria for Serial Dilution Operations on Liquid-Handling Equipment J Biomol Screen, September 1, 2009; 14(8): 1017 - 1030. [Abstract] [PDF]

				W. P. Janzen and I. G. Popa-Burke Review: Advances in Improving the Quality and Flexibility of Compound Management J Biomol Screen, June 1, 2009; 14(5): 444 - 451. [Abstract] [PDF]

				I. Coma, L. Clark, E. Diez, G. Harper, J. Herranz, G. Hofmann, M. Lennon, N. Richmond, M. Valmaseda, and R. Macarron Process Validation and Screen Reproducibility in High-Throughput Screening J Biomol Screen, January 1, 2009; 14(1): 66 - 76. [Abstract] [PDF]

				C. Quintero, C. Rosenstein, B. Hughes, R. Middleton, and I. Kariv Quality Control Procedures for Dose-Response Curve Generation Using Nanoliter Dispense Technologies J Biomol Screen, September 1, 2007; 12(6): 891 - 899. [Abstract] [PDF]

				H. Rhode, M. Schulze, S. Renard, P. Zimmermann, T. Moore, G. A. Cumme, and A. Horn An Improved Method for Checking HTS/uHTS Liquid-Handling Systems J Biomol Screen, December 1, 2004; 9(8): 726 - 733. [Abstract] [PDF]