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A Standard Operating Procedure for Assessing Liquid Handler Performance in High-Throughput Screening

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Stephen Ashman

Department of Assay Methodology Development, New Frontiers Science Park, Harlow, United Kingdom

Stuart M. Baddeley

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Stacy L. Bartram

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Clive D. Battle

Cheminformatics, King of Prussia, PA and Stevenage, United Kingdom

Brian C. Bond

Department of Statistical Sciences, New Frontiers Science Park, Harlow, United Kingdom

Yvonne M. Clements

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Nathan J. Gaul

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

W. Elliot McAllister

Cheminformatics, King of Prussia, PA and Stevenage, United Kingdom

Juan A. Mostacero

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Fernando Ramon

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Jamie M. Wilson

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Robert P. Hertzberg

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

Andrew J. Pope

Systems Research, King of Prussia, PA.

Ricardo Macarron

GlaxoSmithKline Pharmaceuticals, Department of Molecular Screening, King of Prussia, PA and Parque Tecnologico de Madrid, Spain and Stevenage, United Kingdom

The thrust of early drug discovery in recent years has been toward the configuration of homogeneous miniaturized assays. This has allowed organizations to contain costs in the face of exponential increases in the number of screening assays that need to be run to remain competitive. Miniaturization brings with it an increasing dependence on instrumentation, which over the past several years has seen the development of nanodispensing capability and sophisticated detection strategies. To maintain confidence in the data generated from miniaturized assays, it is critical to ensure that both compounds and reagents have been delivered as expected to the target wells. The authors have developed a standard operating procedure for liquid-handling quality control that has enabled them to evaluate performance on 2 levels. The first level provides for routine daily testing on existing instrumentation, and the second allows for more rigorous testing of new dispensing technologies. The procedure has shown itself to be useful in identifying both method programming and instrumentation performance shortcomings and has provided a means to harmonizing instrumentation usage by assay development and screening groups. The goal is that this type of procedure be used for facilitating the exchange of liquid handler performance data across the industry.

Journal of Biomolecular Screening, Vol. 7, No. 6, 554-569 (2002)
DOI: 10.1177/1087057102238630


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