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Journal of Biomolecular Screening
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High-Throughput Screening for Ion Channel Modulators

Margaret Falconer

Lead Discovery Technologies, Pfizer Global Research and Development, Sandwich, United Kingdom

Fiona Smith

Hit Discovery Group, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Sandha Surah-Narwal

Hit Discovery Group, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Gill Congrave

Cardiovascular Biology, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Zhen Liu

Hit Discovery Group, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Paul Hayter

Hit Discovery Group, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Giuseppe Ciaramella

Hit Discovery Group, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Wilma Keighley

Lead Discovery Technologies, Pfizer Global Research and Development, Sandwich, United Kingdom

Peter Haddock

Cardiovascular Biology, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Gareth Waldron

Hit Discovery Group, Pfizer Global Research and Development, Lead Discovery Technologies, Sandwich, United Kingdom

Andreas Sewing

Lead Discovery Technologies, Pfizer Global Research and Development, Sandwich, United Kingdom

Ion channels present a group of targets for major clinical indications, which have been difficult to address due to the lack of suitable rapid but biologically significant methodologies. To address the need for increased throughput in primary screening, the authors have set up a Beckman/Sagian core system to fully automate functional fluorescence-based assays that measure ion channel function. They apply voltage-sensitive fluorescent probes, and the activity of channels is monitored using Aurora's Voltage/Ion Probe Reader (VIPR). The system provides a platform for fully automated high-throughput screening as well as pharmacological characterization of ion channel modulators. The application of voltage-sensitive fluorescence dyes coupled with fluorescence resonance energy transfer is the basis of robust assays, which can be adapted to the study of a variety of ion channels to screen for both inhibitors and activators of voltage-gated and other ion channels.

Journal of Biomolecular Screening, Vol. 7, No. 5, 460-465 (2002)
DOI: 10.1177/108705702237678
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