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Journal of Biomolecular Screening
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Plasmodium falciparum: In Vitro Cytotoxicity Testing Using MTT

J. Enrico Lazaro

Marine Science Institute, University of the Philipines, Diliman 1101, Quezon City, Philippines

Frederick Gay

Service de Parasitologie, Groupe Hospitalier Piti6-Salpetriere, 47 Bd. del H6pital, Paris 75013, France

The microculture tetrazolium assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was used to estimate the 50% inhibitory concentration of chloroquine, quinine, artemisinin, and atovaquone using a Plasmodium falciparum in vitro culture system. The MTT assay was compared to the standard tritiated hypoxan-thine assay and to a previously described method, the 2,2'-di-p-nitrophenyl-5,5'-diphenyl-3,3'-[3,3'-dimethoxy-4,4'-diphenylenel-ditetrazolium chloride (NBT) assay. In general, the results show that the three assays generate comparative results.

The results of this study suggest that the MTT method is able to give a profile of cytotoxic dose response effects over a wide range of concentrations of a drug. The method may be used in work that does not require extreme pre-cision and sensitivity, for instance, as a portable rapid screen to assay natural products for in vitro cytotoxic ac-tivity against Plasmodium falciparum.

Journal of Biomolecular Screening, Vol. 3, No. 1, 49-53 (1998)
DOI: 10.1177/108705719800300107
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