Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biomolecular Screening
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
1087057109336595v1
14/6/662    most recent
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Aneiros, E.
Right arrow Articles by Dabrowski, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aneiros, E.
Right arrow Articles by Dabrowski, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Novel Temperature Activation Cell-Based Assay on Thermo-TRP Ion Channels

Eduardo Aneiros

AstraZeneca R&D, Molecular Pharmacology, Södertälje, Sweden

Michael Dabrowski

AstraZeneca R&D, Molecular Pharmacology, Södertälje, Sweden, michael.a.dabrowski{at}astrazeneca.com

The precise temperature control of the ABI Prism® 7900HT Sequence Detection System designed for detection of fluorescence of a biological sample in real-time PCR assays (TaqMan assays) was used to activate Thermo-TRP ion channels, enabling a novel 384-/96-well plate-based assay. Functional pharmacology was verified against the temperature activation using intracellular calcium fluorescence as a measure of ion channel activity. The assay is applicable to both heterologous expression systems and dorsal root ganglia primary cells. This will benefit several analgesic drug discovery programs searching for new Thermo-TRP modulators. (Journal of Biomolecular Screening 2009:662-667)

Key Words: Thermo-TRP • cell-based assay • temperature activation • dorsal root ganglion cells

This version was published on July 1, 2009

Journal of Biomolecular Screening, Vol. 14, No. 6, 662-667 (2009)
DOI: 10.1177/1087057109336595
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?