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Journal of Biomolecular Screening
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Long-Term Storage of Compound Solutions for High-Throughput Screening by Using a Novel 1536-Well Microplate

Martin Joseph Pfeifer

Novartis Pharma AG, Basel, Switzerland

Guenther Scheel

Novartis Pharma AG, Basel, Switzerland, guenther.scheel{at}novartis.com

This report describes the features and the performance of a new and significantly improved 1536-well microplate design. The design allows for simple, automation-friendly, and cost-effective storage of compound solutions for high-throughput screening. The plate design is based on Society for Biomolecular Sciences standards for microplates and can be molded from polystyrene or cycloolefin copolymer, thus making the plate suitable for use with acoustic dispensing as well as other conventional liquid dispensing in the nanoliter range. For a 9:1 DMSO/water mix as solvent, the novel plate design has shown to perform over 4 months with only minor losses in solvent. Thus, this novel plate design creates the basis for further reductions in compound storage volumes and allows for an increase in the storage times for microliter volumes for up to a year or more. The high protection against solvent evaporation is also visible for aqueous solutions, thus allowing for reduced edge effects during screening campaigns.

Key Words: 1536-well microplate • compound storage • miniaturized liquid handling • ultra-high-throughput screening

This version was published on June 1, 2009

Journal of Biomolecular Screening, Vol. 14, No. 5, 492-498 (2009)
DOI: 10.1177/1087057109335261
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