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Journal of Biomolecular Screening
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PDMS Compound Adsorption in Context

Nianzhen Li

Fluxion Biosciences, South San Francisco, California

Michael Schwartz

Fluxion Biosciences, South San Francisco, California

Cristian Ionescu-Zanetti

Fluxion Biosciences, South San Francisco, California, Cristian.Ionescu-Zanetti{at}fluxionbio.com

Soft lithography of polydimethylsiloxane (PDMS), an elastomeric polymer, has enabled rapid and inexpensive fabrication of microfluidic devices for various biotechnology applications. However, concerns remain about adsorption of compounds on PDMS surfaces because of its porosity and hydrophobicity. Here, the adsorption of 2 small fluorescent dyes of different hydrophobicity (calcein and 5- (and 6-)carboxytetramethylrhodamine (TMR)) on PDMS surface has been systematically characterized, and PDMS adsorption has been compared with 2 traditional substrates: glass and polystyrene. To characterize adsorption in a regimen that is more relevant to microfluidic applications, the adsorption and desorption of the 2 compounds in PDMS microfluidic channels under flow conditions were also studied. Results showed that there was minimal adsorption of the hydrophilic compound calcein on PDMS, whereas the more hydrophobic TMR adsorbed on PDMS up to 4 times of that on glass or polystyrene. Under flow conditions, the desorption profiles and times needed to drop desorbed compound concentrations to negligible levels (desorption time constant, 10-42 s) were characterized. In the worst case scenario, after a 4-min exposure to TMR, 4 min of continuous wash resulted in compound concentrations in the microchannels to drop to values below 2 x 10— 5 of the initial concentration. (Journal of Biomolecular Screening 2009:194-202)

Key Words: microfluidic • flow • compound adsorption • desorption • hydrophobic • fluorescence

This version was published on February 1, 2009

Journal of Biomolecular Screening, Vol. 14, No. 2, 194-202 (2009)
DOI: 10.1177/1087057108327326
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