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Journal of Biomolecular Screening
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Antiretroviral Therapy-Induced Functional Modification of IgG4 and IgM Responses in HIV-1–Infected Individuals Screened by an Allosteric Biosensor

Rosa María Ferraz

Departament de Matemàtica Aplicada IV, Universitat Politècnica de Catalunya, Campus Nord, Barcelona, Spain, Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain, CIBER-BBN en Bioingeniería, Biomateriales y Nanomedicina, Barcelona, Spain

Miguel Angel Martínez

Fundació irsiCaixa, Universitat Autònoma de Barcelona, Hospital Universitari Germans Trias i Pujol, Badalona, Spain

Rafael Cubarsi

Departament de Matemàtica Aplicada IV, Universitat Politècnica de Catalunya, Campus Nord, Barcelona, Spain, CIBER-BBN en Bioingeniería, Biomateriales y Nanomedicina, Barcelona, Spain

Antonio Villaverde

Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain, avillaverde{at}servet.uab.es, CIBER-BBN en Bioingeniería, Biomateriales y Nanomedicina, Barcelona, Spain

We have explored the effect of antiretroviral drugs on the antiviral immune response in human immunodeficiency virus-1 (HIV-1)—infected patients by using an enzymatic immunosensor that detects epitope-modifying anti-gp41 antibodies. By this molecular sensing approach, we have identified an irreversible impact of drug administration on the functionality of IgG4 and IgM specific antibodies regarding the structural modification promoted on their target epitope. During the antiretroviral therapy, the prevalent induced fit promoted by IgM on the epitope was lost at the expense of that promoted by IgG4, suggesting alternative-ness in the neutralization potency of these antibody subpopulations. Because the particular drug composition of the antiretroviral treatment did not affect such immune shift, the obtained data strongly suggest that the drop in the viral load and the consequent lost of antigenemia are responsible for the functional adaptation observed in the humoral response. (Journal of Biomolecular Screening 2008:817-821)

Key Words: antiviral drug • immunosensor • HIV-1 • humoral response • gp41

This version was published on September 1, 2008

Journal of Biomolecular Screening, Vol. 13, No. 8, 817-821 (2008)
DOI: 10.1177/1087057108323126
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