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Journal of Biomolecular Screening
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Profiling Protein Tyrosine Phosphorylation: A Quantitative 45-Plex Peptide-Based Immunoassay

Timothy K. Nadler

Epitome Biosystems, Waltham, Massachusetts

Christine Rauh-Adelmann

Epitome Biosystems, Waltham, Massachusetts

Cheryl Murphy

Epitome Biosystems, Waltham, Massachusetts

Amy B. Hall

Epitome Biosystems, Waltham, Massachusetts

James R. Graham

Epitome Biosystems, Waltham, Massachusetts

Lucy Yen

Epitome Biosystems, Waltham, Massachusetts

Neal F. Gordon

Epitome Biosystems, Waltham, Massachusetts

Jeffrey A. Radding

Epitome Biosystems, Waltham, Massachusetts, jradding{at}epitomebiosystems.com

Cellular homeostasis and responses to stimuli are mediated by complex signaling network events dominated by changes in protein phosphorylation states. Understanding information flow in the network is essential for correlating signaling changes to cell physiology. Tyrosine phosphorylation constitutes only a small portion of all protein phosphorylation, but its importance is manifested by the significant role it plays in diseases such as cancer. A peptide-based immunoassay microarray, designed to provide site specificity, quantification, broad coverage, and accessibility, is described that profiles 45 tyrosine phosphorylation sites across 34 proteins. Epidermal growth factor—stimulated A431 cells in the absence and presence of kinase inhibitors analyzed by microarrays showed biologically validated tyrosine phosphorylation changes and unanticipated activation of other targets. The approach is scalable for increasing the breadth of content as well as for interrogating other types of protein posttranslational modifications. ( Journal of Biomolecular Screening 2008:626-637)

Key Words: tyrosine phosphorylation • signal transduction • antibody microarray • molecular profiling

This version was published on August 1, 2008

Journal of Biomolecular Screening, Vol. 13, No. 7, 626-637 (2008)
DOI: 10.1177/1087057108319978
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