Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biomolecular Screening
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
1087057108317768v1
13/5/354    most recent
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Wigglesworth, M.J.
Right arrow Articles by Wise, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wigglesworth, M.J.
Right arrow Articles by Wise, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Use of Cryopreserved Cells for Enabling Greater Flexibility in Compound Profiling

M.J. Wigglesworth

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK, M.J.Wigglesworth{at}GSK.com

K.J. Lawless

Biopharm Discovery Technology Group

D.J. Standing

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

E.K. Mackenzie

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

V.R. Kitchen

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

F. Mckay

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

E. Ward

Biochemical Cell Targets

S.J. Brough

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

M. Stylianou

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

F.R. Jewitt

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

A.M. Mclaren-Douglas

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

M.I. Jowet

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

N. Tamayama

Biological Reagents and Assay Development, GlaxoSmithKline, Stevenage, Hertfordshire, UK

D. Finnigan

Biological Reagents and Assay Development, GlaxoSmithKline, Stevenage, Hertfordshire, UK

J. Ding

Discovery Statistics, Upper Providence, Collegeville, PA

A. Wise

Screening and Compound Profiling, GlaxoSmithKline, Harlow, UK

Measurement of intracellular calcium release following agonist challenge within cells expressing the relevant membrane protein is a commonly used format to derive structure-activity relationship (SAR) data within a compound profiling assay. The Fluorometric Imaging Plate Reader (FLIPR) has become the gold standard for this purpose. FLIPR traditionally uses cells that are maintained in continuous culture for compound profiling of iterative chemistry campaigns. This supply dictates that assays can only be run on 4 of 5 weekdays, or alternative cell culture machinery is required such that plating can occur remotely at the weekend. The data reported here demonstrate that high-quality compound profiling data can be generated from the use of cryopreserved cells and that these cells can also be plated at various densities to generate equivalent data between 24 and 72 h post-plating. Hence, the authors report a method that allows data generation throughout the week and without the requirement of highly automated cell culture or continuous culture. (Journal of Biomolecular Screening 2008:354-362)

Key Words: cryopreserved • FLIPR • histamine • alpha 1A • alpha 1B

This version was published on June 1, 2008

Journal of Biomolecular Screening, Vol. 13, No. 5, 354-362 (2008)
DOI: 10.1177/1087057108317768
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J Biomol ScreenHome page
H. Wise, P. W. Abel, and D. Cawkill
Use of Reduced Temperature Cell Pausing to Enhance Flexibility of Cell-Based Assays
J Biomol Screen, July 1, 2009; 14(6): 716 - 722.
[Abstract] [PDF]