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This version was published on April 1, 2008
Journal of Biomolecular Screening, Vol. 13, No. 4, 302-308 (2008)
DOI: 10.1177/1087057108316737
© 2008 Society for Biomolecular Sciences

Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe

Zhiliang Jiang

School of Environment and Resource, Guangxi Normal University, Guilin 541004, China, zljiang{at}mailbox.gxnu.edu.cn, Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China

Lili Wei

School of Environment and Resource, Guangxi Normal University, Guilin 541004, China

Mingjing Zou

School of Environment and Resource, Guangxi Normal University, Guilin 541004, China

Aihui Liang

Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China

Mianwu Meng

School of Environment and Resource, Guangxi Normal University, Guilin 541004, China

Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH2PO 4-Na2HPO4 buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm ({Delta}I580 nm) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity. (Journal of Biomolecular Screening 2008:302-308)

Key Words: immunoglobulin M • nanogold-labeled • resonance scattering • spectral probe • immunoassay


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