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Journal of Biomolecular Screening
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Mouse Thymus Targeted Peptide Isolated by In Vivo Phage Display Can Inhibit Bioactivity of Thymus Output In Vivo

Yang Yu

Department of Gene Detection, Nanjing Clinical Nuclear Medicine Center, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing, Jiangsu, China

Zizheng Wang

Department of Nuclear Medicine, Nanjing Clinical Nuclear Medicine Center, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing, Jiangsu, China, zzwang136{at}yahoo.com.cn

Tongxin Du

Department of Immunology, Nanjing Clinical Nuclear Medicine Center, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing, Jiangsu, China

Heterogeneity of the vasculature in different organs has been well documented by the method of in vivo phage display. Using this technology, several peptide ligands that home to tissue-specific vascular endothelial cell have been isolated. Such peptide ligands directed against specific vascular surface molecules can be used as targeted therapeutic compounds or imaging agents to the vasculature of the specific organ in vivo. In this study, the authors perform in vivo selection in mice using a phage display random peptide library and separated phage peptides homing to mouse thymus by 3 rounds of in vivo panning. Sequence analysis showed that CHAQGSAEC is the dominant peptide sequence. Immunohistochemistry confirmed that the phage peptide CHAQGSAEC can bind specifically to thymus blood vessels in mice. Furthermore, phage peptide CHAQGSAEC and free peptide CHAQGSAEC can inhibit the bioactivity of thymus output in vivo. These results indicate the feasibility of the targeted peptide for possible function as a kind of tool to inhibit thymus bioactivity or as a targeted compound for targeted medicine. (Journal of Biomolecular Screening 2008:968-974)

Key Words: in vivo • phage display • phage peptide • thymus • thymus output

This version was published on December 1, 2008

Journal of Biomolecular Screening, Vol. 13, No. 10, 968-974 (2008)
DOI: 10.1177/1087057108326537
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