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Journal of Biomolecular Screening
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Plaque-Based Competitive Hybridization

Zoltán Villányi

Institute of Genetics, Agricultural Biotechnology Center, Gödöllö, Hungary, Present address: Faculty of General Medicine, Department of Biology, University of Szeged, Szeged, Hungary

István Gyurján

Institute of Genetics, Agricultural Biotechnology Center, Gödöllö, Hungary, Department of Genetics, Faculty of Science, Eötvös Loránd Science University and Research Group for Molecular Genetics of the Hungarian Academy of Sciences, Pázmány P. I/C, 1117 Budapest, Hungary

Viktor Stéger

Institute of Genetics, Agricultural Biotechnology Center, Gödöllö, Hungary, Department of Genetics, Faculty of Science, Eötvös Loránd Science University and Research Group for Molecular Genetics of the Hungarian Academy of Sciences, Pázmány P. I/C, 1117 Budapest, Hungary

László Orosz

Institute of Genetics, Agricultural Biotechnology Center, Gödöllö, Hungary, orosz{at}abc.hu, Department of Genetics, Faculty of Science, Eötvös Loránd Science University and Research Group for Molecular Genetics of the Hungarian Academy of Sciences, Pázmány P. I/C, 1117 Budapest, Hungary

The authors have developed a simple, cost-saving experimental design, plaque-based competitive hybridization (PBCH), for genome-wide identification of genes differentially expressed in different tissues. PBCH offers advantages in comparison with other methods used in comparative genomics by combining the principles of differential hybridization with the subtractive hybridization. PBCH is particularly advantageous when libraries with few differences are to be analyzed. The authors demonstrate the use of PBCH by identifying 3 genes, up-regulated in the developing velvet antler of red deer (Cervus elaphus): ApoD, C011A2, and S100a1. The fidelity and sensitivity of PBCH is also shown: 1 specific clone among a library sample of 15,000 can be recognized. Possibilities for further utilizations are discussed. (Journal of Biomolecular Screening 2008:80-84)

Key Words: deer • bone • cartilage • antler • library screening

Journal of Biomolecular Screening, Vol. 13, No. 1, 80-84 (2008)
DOI: 10.1177/1087057107310876
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