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Journal of Biomolecular Screening, Vol. 12, No. 7, 999-1005 (2007)
DOI: 10.1177/1087057107306502
© 2007 Society for Biomolecular Sciences

Image-Based High-Throughput Quantification of Cellular Fat Accumulation

Mike Dragunow

Department of Pharmacology, University of Auckland, New Zealand, The National Research Centre for Growth and Development, Faculty of Medical and Health Sciences, University of Auckland, New Zealand, m.dragunow{at}auckland.ac.nz

Rachel Cameron

Department of Pharmacology, University of Auckland, New Zealand

Pritika Narayan

Department of Pharmacology, University of Auckland, New Zealand, The National Research Centre for Growth and Development, Faculty of Medical and Health Sciences, University of Auckland, New Zealand

Simon O'Carroll

Department of Anatomy, University of Auckland, New Zealand

A number of biochemical methods are available for measuring fat accumulation in cell culture. The authors report a simple image-based method for measuring fat accumulation in adipocytes using a combination of high-throughput brightfield microscopy and image analysis, which was validated biochemically using Oil-Red-O. The quickest and most accurate method of analysis was one based on thresholding brightfield images and determining the area of fat droplets per image. Thus, the authors have developed a simple high-throughput, label-free method for measuring fat accumulation that is applicable to any cell or tissue type where fat droplets are visible under light microscopy. (Journal of Biomolecular Screening 2007:999-1005)

Key Words: image analysis • lipids • high-content screening


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