Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biomolecular Screening
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Rodi, D. J.
Right arrow Articles by Fischetti, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodi, D. J.
Right arrow Articles by Fischetti, R. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Detection of Functional Ligand-Binding Events Using Synchrotron X-Ray Scattering

Diane J. Rodi

Biosciences Division, Argonne National Laboratory, Argonne, Illinois, drodi{at}anl.gov

Suneeta Mandava

Biosciences Division, Argonne National Laboratory, Argonne, Illinois

David B. Gore

BioCAT, CSRRI, and BCPS, Illinois Institute of Technology, Chicago

Lee Makowski

Biosciences Division, Argonne National Laboratory, Argonne, Illinois

Robert F. Fischetti

Biosciences Division, Argonne National Laboratory, Argonne, Illinois, GM/CA CAT, Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois

Small-molecule ligands that change the structure of a protein are likely to affect its function, whereas those causing no structural change are less likely to be functional. Wide-angle x-ray scattering (WAXS) can be easily carried out on proteins and small molecules in solution in the absence of chemical tags or derivatives. The authors demonstrate that WAXS is a sensitive probe of ligand binding to proteins in solution and can distinguish between nonfunctional and productive binding. Furthermore, similar ligand-binding modes translate into similar scattering patterns. This approach has high potential as a novel, generic, low-throughput assay for functional ligand binding. (Journal of Biomolecular Screening 2007:994-998)

Key Words: ligand binding • wide-angle x-ray scattering

Journal of Biomolecular Screening, Vol. 12, No. 7, 994-998 (2007)
DOI: 10.1177/1087057107306104
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?