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Journal of Biomolecular Screening
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A High-Throughput Soft Agar Assay for Identification of Anticancer Compound

Steven N. Anderson

Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL

Danli L. Towne

Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL

David J. Burns

Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL

Usha Warrior

Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL, usha.warrior{at}abbott.com

A 384-well soft agar assay was developed to identify potential novel anticancer compounds. Normally used to detect cell transformation, the assay is used here to quantitate cell proliferation in a 3-dimensional (3-D) anchorage-independent format. HCC827 cells, which are highly sensitive to epithelial growth factor receptor (EGFR) tyrosine kinase inhibitors, were used to develop the method and a set of 9600 compounds used to validate the assay. Results were compared to a monolayer assay using the same compound set. The assay provides a robust method to discover compounds that could be missed using traditional monolayer formats. (Journal of Biomolecular Screening 2007:938-945)

Key Words: soft agar • HTS • 3-D • anoikis • HCC827

Journal of Biomolecular Screening, Vol. 12, No. 7, 938-945 (2007)
DOI: 10.1177/1087057107306130
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