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This version was published on June 1, 2007
Journal of Biomolecular Screening, Vol. 12, No. 4, 568-577 (2007)
DOI: 10.1177/1087057107301007
© 2007 Society for Biomolecular Sciences

Rapid Screen of Human Genes for Relevance to Cancer Using Fission Yeast

Kyung-Sook Chung

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Young-Joo Jang

School of Dentistry, Dankook University, Cheonan, Korea

Nam-Soon Kim

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Sun-Yong Park

Laboratory of Human Genome, KRIBB, Daejeon, Korea, College of Agriculture and Life Science and Chungnam National University, Daejeon, Korea

Shin-Jung Choi

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Ji-Youn Kim

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Ji-Hee Ahn

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Hyun-Ji Lee

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Ji-Hyun Lim

College of Agriculture and Life Science and Chungnam National University, Daejeon, Korea

Ju-Hyun Song

College of Agriculture and Life Science and Chungnam National University, Daejeon, Korea

Jae-Hoon Ji

College of Agriculture and Life Science and Chungnam National University, Daejeon, Korea

Jung-Hwa Oh

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Kyung Bin Song

College of Agriculture and Life Science and Chungnam National University, Daejeon, Korea

Hyang-Sook Yoo

Laboratory of Human Genome, KRIBB, Daejeon, Korea

Misun Won

Laboratory of Human Genome, KRIBB, Daejeon, Korea, misun{at}kribb.re.kr

A total of 437 human full-length cDNAs isolated by microarray analysis of liver and/or gastric cancer tissues were evaluated for their relevance to cancer using the fission yeast Schizosaccharomyces pombe. Overexpression of 161 human cDNAs in S. pombe caused growth inhibition and/or morphological changes, which can be considered as cancer-related phenotypes of S. pombe. Sixteen genes causing growth defects and morphological changes at the same time were chosen to validate their ostensible oncogenic properties. They were highly expressed in liver and/or gastric cancer cell lines. Also, when the mouse embryonic fibroblast cell type NIH3T3 was transfected with these genes, the proliferation rates of cells were increased by 32% to 120%. This study demonstrates that fission yeast can be used as an advantageous and powerful tool for the rapid screening of human genes relevant to cancer. Furthermore, the human genes screened can be tested further as diagnostic markers and potential therapeutic targets for liver and stomach cancers. They also can be studied further for the elucidation of mechanisms involved in carcinogenesis. (Journal of Biomolecular Screening 2007:568-577)

Key Words: cell-based assay • overexpression • oncogenesis • phenotype • fission yeast


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