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Quality Assessment and Analysis of Biogen Idec Compound Library

Biogen Idec, Cambridge, MA, Novartis Institutes for BioMedical Research, Cambridge, MA

Dongyu Sun

Biogen Idec, Cambridge, MA

Azita Kaffashan

Biogen Idec, Cambridge, MA, Dionex Corporation, Sunnyvale, CA

Chenhui Zeng

Biogen Idec, Cambridge, MA, Beyond Genomics Medicine, Waltham, MA

Claudio Chuaqui

Biogen Idec, Cambridge, MA

Xiaoping Hronowski

Biogen Idec, Cambridge, MA

Alex Buko

Biogen Idec, Cambridge, MA

Xin Zhang

Biogen Idec, Cambridge, MA

Serene Josiah

Biogen Idec, Cambridge, MA

A subset of the compound repository for lead identification at Biogen Idec was characterized for its chemical stability over a 3-year period. Compounds were stored at 4 °C as 10 mM DMSO stocks, and a small subset of compounds was stored as lyophilized dry films. Compound integrity of 470 discrete compounds (Compound Set I) and 1917 combinatorial chemistry-derived compounds (Compound Set II) was evaluated by liquid chromatography/mass spectrometry from the time of acquisition into the library collection and after 3 years of storage. Loss of compound integrity over the 3 years of storage was observed across the 2 subsets tested. Of Compound Set I, 63% of samples retained > 80% purity, whereas 57% of samples from Compound Set II had purity greater than 60%. The stability of the lyophilized samples was superior to the samples stored as DMSO solution. Although storage at 4 °C as DMSO solution was adequate for the majority of compounds, the authors observed and quantified the level of degradation within the compound collection. Their study provides general insight into compound storage and selection of library subsets for future lead identification activities.

Key Words: compound management • compound purity analysis • compound precipitation • chemical stability • LC/MS

This version was published on October 1, 2006

Journal of Biomolecular Screening, Vol. 11, No. 7, 828-835 (2006)
DOI: 10.1177/1087057106290993


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