Journal of Biomolecular Screening

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
1087057106286654v1
11/4/369    most recent
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, L.
Right arrow Articles by Ma, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, L.
Right arrow Articles by Ma, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
This version was published on June 1, 2006
Journal of Biomolecular Screening, Vol. 11, No. 4, 369-376 (2006)
DOI: 10.1177/1087057106286654

Cell-Based Screening and Validation of Human Novel Genes Associated with Cell Viability

Lan Wang

Chinese National Human Genome Center, Beijing, China, Lab of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China, Human Disease Genomics Center, Peking University, Beijing, China

Xia Gao

Chinese National Human Genome Center, Beijing, China

Peng Gao

Chinese National Human Genome Center, Beijing, China

Weiwei Deng

Chinese National Human Genome Center, Beijing, China

Peng Yu

Lab of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China, Human Disease Genomics Center, Peking University, Beijing, China

Jinjing Ma

Chinese National Human Genome Center, Beijing, China

Jinhai Guo

Chinese National Human Genome Center, Beijing, China

Xinyu Wang

Chinese National Human Genome Center, Beijing, China

Hualing Cheng

Chinese National Human Genome Center, Beijing, China

Chenying Zhang

Chinese National Human Genome Center, Beijing, China

Chuanfei Yu

Lab of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China, Human Disease Genomics Center, Peking University, Beijing, China

Xi Ma

Lab of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China, Human Disease Genomics Center, Peking University, Beijing, China

Bingfeng Lv

Lab of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China, Human Disease Genomics Center, Peking University, Beijing, China

Yang Lu

Chinese National Human Genome Center, Beijing, China

Taiping Shi

Chinese National Human Genome Center, Beijing, China

Dalong Ma

Chinese National Human Genome Center, Beijing, China, Lab of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China, Human Disease Genomics Center, Peking University, Beijing, China

In the present study, a cell-based high-throughput assay is established to identify novel human genes associated with cell viability. The assay relies on the down-regulation of Renilla luciferase (pRL) activity in a 96-well format. In addition, 2-color fluorescence probes were used to distinguish living and dead cells. As the positive control, the authors used the expression vectors encoding Bax, TNFRSF1A, and TAJ, which were widely known to effectively induce programmed cell death. They screened 409 novel genes (including alternative mRNA splicing forms) cloned in their laboratory and found that 39 genes could significantly down-regulate pRL activity. A subsequent fluorescence-based assay revealed that 4 of the 39 genes (PIP5KL1, OLFM1, RNF122, FAM26B) were associated with cell viability. Further function assays validated that the 4 genes were able to induce both necrosis and apoptosis. These results therefore indicate that a rapid and effective screening system has been developed, which should shed light on some functions of novel genes.

Key Words: high-throughput screening • fluorescence • cell viability • novel gene • imaging


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?