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NanoStore: A Concept for Logistical Improvements of Compound Handling in High-Throughput Screening

Discovery Biology, Pfizer Global Research and Development, Ramsgate Road, Sandwich, CT13 9NJ, United Kingdom

Helen F. Boyd

Discovery Biology, Pfizer Global Research and Development, Ramsgate Road, Sandwich, CT13 9NJ, United Kingdom

Jeremy R. Everett

Medicinal Technologies, Pfizer Global Research and Development, Ramsgate Road, Sandwich, CT13 9NJ, United Kingdom

Joachim Fries

Drug Discovery Services, Evotec OAI, Hamburg, FRG

Philip Gribbon

Medicinal Technologies, Pfizer Global Research and Development, Ramsgate Road, Sandwich, CT13 9NJ, United Kingdom

Nuzrul Haque

Discovery Biology, Pfizer Global Research and Development, Ramsgate Road, Sandwich, CT13 9NJ, United Kingdom

Karsten Henco

Drug Discovery Services, Evotec OAI, Hamburg, FRG

Timm Jessen

Drug Discovery Services, Evotec OAI, Hamburg, FRG

William H. Martin

Exploratory Medicinal Sciences, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340

Travis J. Mathewson

Exploratory Medicinal Sciences, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340

R. Eryl Sharp

Exploratory Medicinal Sciences, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340

Robin W. Spencer

Exploratory Medicinal Sciences, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340

Frank Stuhmeier

Discovery Biology, Pfizer Global Research and Development, Ramsgate Road, Sandwich, CT13 9NJ, United Kingdom

Mark S. Wallace

Exploratory Medicinal Sciences, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340

Dirk Winkler

Drug Discovery Services, Evotec OAI, Hamburg, FRG

Small molecule screening, the systematic encounter of biology space with chemical space, has provoked the emergence of a whole industry that recreates itself by constant iterative improvements to this process. The authors describe an approach to tackle the problem for one of the most time-consuming steps in the execution of a screening campaign, namely, the reformatting of high-throughput screening test compounds from master plates to daughter assay plates used in the execution of the screen. Through an engineered storage procedure, they prepare plates ahead of the screening process with the respective compounds in a ready-to-use format. They show the biological inertness of the method and how it facilitates efficient recovery of compound activity. This uncoupling of normally interconnected processes provides time and compound savings, avoids repeated freeze-thaw cycles of compound solutions, and removes the problems associated with the DMSO sensitivity of certain assays types.

Key Words: assay miniaturization • ultra-high-throughput screening • compound storage and recovery • 1536-well and 2080-well NanoCarriers^TM

This version was published on September 1, 2005

Journal of Biomolecular Screening, Vol. 10, No. 6, 573-580 (2005)
DOI: 10.1177/1087057105277234


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