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Journal of Biomolecular Screening
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Two-Photon Excitation in Fluorescence Polarization Receptor-Ligand Binding Assay

Marko E. Tirri

Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland

Roope J. Huttunen

Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland; Department of Anatomy, Institute of Biomedicine, University of Turku, Finland

Juha Toivonen

Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland

Pirkko L. Härkönen

Department of Anatomy, Institute of Biomedicine, University of Turku, Finland

Juhani T. Soini

ArcDia Ltd, Arctic Diagnostics Oy, Turku, Finland

Pekka E. Hänninen

Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland

Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

Key Words: two-photon excitation • fluorescence polarization • high-throughput screening • estrogen receptor • drug discovery

Journal of Biomolecular Screening, Vol. 10, No. 4, 314-319 (2005)
DOI: 10.1177/1087057104273334
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