This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (19) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gribbon, P. Articles by Sewing, A. Search for Related Content PubMed PubMed Citation Articles by Gribbon, P. Articles by Sewing, A. Pubmed/NCBI databases Medline Plus Health Information Health Statistics Social Bookmarking                         What's this?

Evaluating Real-Life High-Throughput Screening Data

Automated Screening Technologies, Pfizer Global Research and Development, Sandwich, UK

Richard Lyons

Nonclinical Statistics, Pfizer Global Research and Development, Sandwich, UK

Philip Laflin

Data Support and Management Pfizer Global Research and Development, Sandwich, UK

Joe Bradley

Automated Screening Technologies, Pfizer Global Research and Development, Sandwich, UK

Chris Chambers

Automated Screening Technologies, Pfizer Global Research and Development, Sandwich, UK

Bruce S. Williams

Data Support and Management Pfizer Global Research and Development, Sandwich, UK

Wilma Keighley

Automated Screening Technologies, Pfizer Global Research and Development, Sandwich, UK

Andreas Sewing

Automated Screening Technologies, Pfizer Global Research and Development, Sandwich, UK

High-throughput screening (HTS) is the result of a concerted effort of chemistry, biology, information technology, and engineering. Many factors beyond the biology of the assay influence the quality and outcome of the screening process, yet data analysis and quality control are often focused on the analysis of a limited set of control wells and the calculated values derived from these wells. Taking into account the large number of variables and the amount of data generated, multiple views of the screening data are necessary to guarantee quality and validity of HTS results. This article does not aim to give an exhaustive outlook on HTS data analysis but tries to illustrate the shortfalls of a reductionist approach focused on control wells and give examples for further analysis.

Key Words: high-throughput screening • data analysis • assay development • biological screening

Journal of Biomolecular Screening, Vol. 10, No. 2, 99-107 (2005)
DOI: 10.1177/1087057104271957


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				I. Burr, T. Winchester, W. Keighley, and A. Sewing Compound Management Beyond Efficiency J Biomol Screen, June 1, 2009; 14(5): 485 - 491. [Abstract] [PDF]

				I. Coma, L. Clark, E. Diez, G. Harper, J. Herranz, G. Hofmann, M. Lennon, N. Richmond, M. Valmaseda, and R. Macarron Process Validation and Screen Reproducibility in High-Throughput Screening J Biomol Screen, January 1, 2009; 14(1): 66 - 76. [Abstract] [PDF]

				L. M. Mayr and P. Fuerst The Future of High-Throughput Screening J Biomol Screen, July 1, 2008; 13(6): 443 - 448. [Abstract] [PDF]

				A. Visegrady, A. Boros, Z. Nemethy, B. Kiss, and G. M. Keseru Application of the BD ACTOneTM Technology for the High-Throughput Screening of Gs-Coupled Receptor Antagonists J Biomol Screen, December 1, 2007; 12(8): 1068 - 1073. [Abstract] [PDF]

				P. Gribbon, C. Chambers, K. Palo, J. Kupper, J. Mueller, and A. Sewing A Novel Method for Analyzing [Ca2+] Flux Kinetics in High-Throughput Screening J Biomol Screen, August 1, 2006; 11(5): 511 - 518. [Abstract] [PDF]

				J. Inglese, D. S. Auld, A. Jadhav, R. L. Johnson, A. Simeonov, A. Yasgar, W. Zheng, and C. P. Austin Quantitative high-throughput screening: A titration-based approach that efficiently identifies biological activities in large chemical libraries PNAS, August 1, 2006; 103(31): 11473 - 11478. [Abstract] [Full Text] [PDF]