This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Oldenburg, K. R. Articles by Yuan, Z. Search for Related Content PubMed Articles by Oldenburg, K. R. Articles by Yuan, Z. Social Bookmarking                         What's this?

A Dual Culture Assay for Detection of Antimicrobial Activity

Affymax Research Institute, 4001 Mindra Avenue, Palo Alto, California 94304

Kham T. Vo

Affymax Research Institute, 4001 Mindra Avenue, Palo Alto, California 94304

Beatrice Ruhland

Affymax Research Institute, 4001 Mindra Avenue, Palo Alto, California 94304

Peter J. Schatz

Affymax Research Institute, 4001 Mindra Avenue, Palo Alto, California 94304

Zhengyu Yuan

Affymax Research Institute, 4001 Mindra Avenue, Palo Alto, California 94304

Combinatorial chemistry has opened a new realm of chemical diversity in the search for useful therapeutics as well as the ability to generate chemical libraries of hundreds of thousands to millions of discrete compounds. For the biologist, the goal is to screen these large libraries quickly and to obtain as much information in the primary screen as possible. Ideally, a primary screen would not only identify potential lead compounds but also yield information about the specificity, toxicity, and potency of that compound. Toward this end, a primary screen has been developed in which two organisms are cocultured, either bacteria, yeast, or mammalian cells, in the presence of a combinatorial library. For example, bacteria and yeast are cocultured either in liquid or in agar. When exposed to compounds from the combinatorial library, individual compounds are found which inhibit bacterial growth antibacterialls, inhibit yeast growth (antifungals), or inhibit both (potential toxins). This screening method is simple, rapid, and eliminates many of the false positives usually encountered in antimicrobial screening.

Journal of Biomolecular Screening, Vol. 1, No. 3, 123-130 (1996)
DOI: 10.1177/108705719600100305


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?