Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biomolecular Screening
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Brann, M. R.
Right arrow Articles by Lannigan, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Brann, M. R.
Right arrow Articles by Lannigan, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Cell-Based Assays for G-Protein-Coupled/Tyrosine Kinase-Coupled Receptors

Mark R. Brann

Receptor Technologies Inc., 276 East Allen, Winooski, Vermont 05404

Terri Messier

Receptor Technologies Inc., 276 East Allen, Winooski, Vermont 05404

Christine Dorman

Receptor Technologies Inc., 276 East Allen, Winooski, Vermont 05404

Deborah Lannigan

Receptor Technologies Inc., 276 East Allen, Winooski, Vermont 05404

Using mammalian cells transiently transfected with receptors, we have developed an assay [Receptor Selection and Amplification Technology (R-SAT); patents pending] that links ligand-dependent cellular transformation to induction of 8-galactosidase in a 96-well plate format. Using these procedures, we have performed high throughput functional assays of receptors that mediate signal transduction by a diversity of mechanisms. Examples include the prostanoid, muscarinic, and neurokinin receptor subtypes that signal via the G-proteins Gq and Gi, the JAK/STAT-linked GM-CSF receptor, the tyrosine kinase neurotrophin receptors, and the classical oncogenes v-ras and p53. The assays have been formatted such that many receptors can be assayed simultaneously (>10 receptors per well) and precise discrimination of ligand efficacy can be obtained (e.g., full and partial agonists, negative and neutral antagonists).

Journal of Biomolecular Screening, Vol. 1, No. 1, 43-45 (1996)
DOI: 10.1177/108705719600100114
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. L. Boger, J. E. Patterson, and Q. Jin
Structural requirements for 5-HT2A and 5-HT1A serotonin receptor potentiation by the biologically active lipid oleamide
PNAS, April 14, 1998; 95(8): 4102 - 4107.
[Abstract] [Full Text] [PDF]

Home page
 J Biomol ScreenHome page
D. S. Gembitsky, S. Lovas, and R. F. Murphy
Development of a High Throughput Functional Assay for Structure-Activity Studies of Neurokinin A Analogs
J Biomol Screen, April 1, 1998; 3(3): 183 - 188.
[Abstract] [PDF]